reassigned to group 5. Figure 5 displays the gene order of the mtDNA from the five revised groups. The difference in the gene order between these two groups is an inversion of two pairs of genes. In group 3, the fifth to the second last genes are NAD6, tRNA-Glu, cytochrome B, and tRNA-Thr. In group 4, the order of these genes is cytochrome B, rTNA-Thr, NAD6, and tRNA-Glu. Furthermore, the mean mtDNA length of the species displayed in Figure 5 is 16,686 bp, whereas the mean mtDNA length in group 4 is 17,713 bp. Cytochrome b is also missing from Myrichthys maculosus in this group. Cyema atrum is another anomalous species, as the length of its mitogenome places it in group 4, whereas its gene order is similar to that of group 3. As to whether there are four or five holobaramins within the Anguilliformes apobaramin, more study is needed. The larger cluster of 41 species also has a relatively low normalized entropy value of 0.485, with 84.1% of the species living in saltwater. This cluster is made up of 36 genera, listed in Supplementary File 2. The smaller cluster consists of species from the genus Anguilla. It is more balanced, with an equal number of species (14) inhabiting all three aquatic environments. Here the normalized Shannon entropy is the highest (0.946). This gives more evidence that the species in this cluster have a better potential to adapt to different water salinities (see Figure 4C). All 14 Anguilla species studied here are catadromous (meaning that they migrate to the sea to spawn). 3. Beloniformes Figure 5. Gene order map of species from several groups from the mtDNA analysis of Anguilliformes. CSERHATI Molecular baraminology of fish 2023 ICC 189
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